Invitrogen bac to bac manual

Bac-to-Bac™ TOPO™ Cloning Kit USER GUIDE Five-minute cloning of blunt PCR products for expression in insect cells Catalog Number A, A, A, A, A Publication Number MAN Revision B.0File Size: 1MB. User Manual Corporate Headquarters Invitrogen Corporation Faraday Avenue Carlsbad, CA T: 1 7F: 1 7E: tech_support@www.doorway.ru For country-specific contact information visit our web site at www.doorway.ru BAC (Bacterial Artificial Chromosome) Clone Collections Catalog nos. , D, , RPCIC,File Size: KB. Bac-to-Bac method delivers high baculovirus titers. Traditional baculovirus systems require purification and amplification of an initial low-titer viral supernatant. In contrast, the Bac-to-Bac Baculovirus Expression System is faster and easier because the technology relies on generation of recombinant baculovirus by site-specific transposition in E. coli rather than homologous .

TOPO® vector are provided in the Bac-to-Bac® TOPO® Cloning Kit manual (part no. A) supplied with the Bac-to-Bac ® HBM TOPO Secreted Expression System. The Bac-to-Bac ® TOPO Cloning Kit manual is also available for downloading at www.doorway.ru or from Technical Support (see page 56). Continued on next page. Bac-to-Bac™ TOPO™ Cloning Kit USER GUIDE Five-minute cloning of blunt PCR products for expression in insect cells Catalog Number A, A, A, A, A Publication Number MAN Revision B Bac-to-Bac™ Vector Kit pFastBac™1 20 µL at µg/µL in TE, pH (10 µg total) pFastBac™-Gus 20 µL at ng/µL in TE, pH (4 ng total) Bac-to-Bac™ HT Vector Kit pFastBac™HT A pFastBac™HT B pFastBac™HT C 20 µL each at µg/µL in TE, pH (10 µg total of each vector) pFastBac™HT-CAT 15 µL at 1 ng/µL in TE, pH

Quick rundown of the bac-to-bac system. I've been working with this system for the past several weeks in my rotation. To generate recombinant baculovirus using the flashBAC™ System, insect cells are Transient plasmid DNA transfection protocol per well of a 6-well plate. Aug Bac-to-Bac® Baculovirus Expression System. An efficient site-specific transposition system to generate baculovirus for.